Wine Cap or Garden Giant Mushroom (Stropharia rugosoannulata) Liquid Mycelium in a Syringe 10ml


Liquid mycelium is intended for use in propagating mycelium on grain or sawdust

Out of stock



Sterile syringe with 10ml mycelium with Luer Lock cap. For use on sterile grain or sawdust.

Store the mycelium at a temperature of +1 – +3 C.

Why use a syringe? The mycelium is in a very sterile environment and it is very convenient to transfer it to another sterile application. Faster colonization and can be used on agar plates.


Weight 0.05 kg
Dimensions 15 × 3 × 3 cm


Growing instructions


Liquid mycelium is one of the best and fastest ways to “seed” mushroom growing substrates and grains. It is a mixture of sterilized water, malt extracts and live mycelium.

Why use liquid culture? Using a syringe, live mycelium can be easily injected (inoculated) into the substrate of choice, reducing the risk of contamination as the mycelium comes from a sterile environment and is injected into a new, also sterile, environment. In addition, the incubation time is significantly reduced and the colonization of the substrate or grain is faster.

How to store liquid mycelium? Store in the refrigerator at a temperature of 1-5°C. Unopened syringes of liquid mycelium can be stored in the refrigerator for three months, but not in the refrigerator for only one month.


Inoculation of grains with liquid mycelium

Cereal grains such as rye, wheat, oats, etc. are suitable for cultivation. Soak them for at least 12 hours before cooking. Then boil for 10-15 minutes. Be careful not to overcook the grains as they spoil more easily. Drain well after cooking. Place the prepared grains in filter bags, which you can also purchase from us, and sterilize in a pressure cooker. To do this, boil them for 1.5 hours at 15 PSI. When they are cooled, take them out and tape them up. Begin inoculation with liquid mycelium. First, you need to disinfect the selected puncture sites (spirit 70%, hydrogen peroxide). Then we inoculate the grain in several places in the bags and seal these places tightly with adhesive tape. After vaccination, place the grain bags in a room with a temperature of around 21°C. Space them out to allow air to circulate between them. After about 10 days, the colonization process will be visible. To speed up the further process, gently mix the grains in the bag.

One syringe can be enough for several bags of grain.

IMPORTANT! In both cases of vaccination, special attention should be paid to the moment of unscrewing the cap and screwing in the needle, when it is necessary to thoroughly disinfect the cap and the thread of the syringe. If we don’t, the negative pressure created by unscrewing the cap can attract bacteria or other airborne fungi into the syringe and contaminate our culture. If you haven’t used all the mycelium, don’t remove the needle, put it back in the bag and refrigerate.


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